反相高效液相色谱法测定雪莲果叶中的绿原酸

采用反相高效液相色谱法对雪莲果叶中的有效成分绿原酸进行分离，并对其含量进行测定，建立反相高效液相色谱法测定雪莲果叶中绿原酸含量的方法。色谱柱为Hypersil ODS-2 C18 柱(4.6mm × 300mm，5μm)，流动相为乙腈- 体积分数0.5% 磷酸溶液(8:92，V/V)，流速为1.0mL/min，检测波长为325nm，柱温为25℃。绿原酸在10～100μg/mL 范围内呈良好线性关系，相关系数为0.9999，加标回收率为97.0%，RSD 为1.10%。本法快速、简单、准确、分离度好，可用于实际样品的检测。

Reversed-Phase High Performance Liquid Chromatographic Determination of Chlorogenic Acid in Yacon Leaves

A method using reversed-phase high performance liquid chromatography was developed for the determination of chlorogenic acid in yacon leaves, an active substance. The chromatographic separation was achieved on a Hypersil ODS column (4.6 mm × 300 mm, 5 μm) using a mobile phase composed of acetonitrile and 0.5% phosphoric acid (8:92, V/V) at a flow rate of 1.0 mL/min. The detection wavelength and the column temperature were set at 325 nm and 25 ℃, respectively. There was a good linear relationship between peak area and chlorogenic acid concentration over the range of 10－100 μg/mL (r = 0.9999), and the average spike recovery for chlorogenic acid was 97.0% with a relative standard deviation (RSD) of 1.10%. The developed method presents the benefits of rapidity, simplicity, high accuracy and good separation.