骨骼肌源性抑瘤物对恶性肿瘤抑制作用的研究

目的研究骨骼肌微环境因素对恶性肿瘤细胞增殖的影响,评价其在骨骼肌转移瘤罕见性中的意义。方法原代培养新生Wistar大鼠骨骼肌细胞,用MTT法分析、比较其条件性上清液(MMCM)对恶性肿瘤细胞(小鼠骨髓瘤细胞株SP2/0、鼻咽癌细胞株CNE、肺巨细胞癌细胞株PLA801C、人食管鳞癌细胞株Eca109、宫颈癌细胞株Hela)及正常非肿瘤细胞(金黄地鼠肾细胞株BHK-21)的体外抑瘤作用。结果MMCM分别与SP2/0、CNE、PLA801C、Eca109、Hela及BHK-21细胞共同培养后,对5株恶性肿瘤细胞的增殖均有明显抑制作用,而正常非肿瘤细胞株则未受影响。结论新生大鼠骨骼肌细胞可产生选择性抑制恶性肿瘤细胞增殖的抑瘤物质,这种抑瘤物质可能是临床上骨骼肌内罕见转移瘤现象的生物学基础。

Suppressive Effect of Skeletal Muscle-derived Tumor Inhibitor on Maligant Tumors

Objective To explore the organic microenvironmental effect of skeletal muscles on the proliferation of malignant cells and evaluate its significance in the rarity of metastases in skeletal muscles.Methods Prepare the primary culture of new born Wistar rat skeletal muscle cells and collect the culture supernatant as murine skeletal muscle conditioned medium(MMCM).Analyze the suppressive effect in vitro of MMCM on the proliferation of malignant tumor cells including murine myeloma cell strain SP2/0,nasopharyngeal carcinoma cell strain CNE,pulmonary cytomegalic carcinoma cell strain PLA-801C,human oesophageal squamous cell carcinoma strain Eca109 and cervical carcinoma cell strain Hela.Results MMCM showed significant suppressive effect on the proliferations of all the five tumor cell strains.However,it showed no significant effect on normal cells.Conclusion The tumor inhibitors derived from new born Wistar rat skeletal muscle cells selectively suppressed the proliferation of malignant tumors and provided a biological basis for the study on rare metastases in skeletal muscles.