新型凹土基益生菌乳化液制备研究

以凹土、大豆分离蛋白、植物油及吐温80制备乳酸杆菌乳化液,通过比较包埋效果可知,油包水型比水包油型乳化液效果好。采用油包水型乳化液时,经正交试验优化后包埋率最佳为92.31%,活菌数为5.171×1010 CFU/mL,乳化性为2.176。乳化液中的乳酸杆菌在胃中随着时间的增加有所下降,在肠中不断释放,刚开始随着时间的增加而增加,于第6小时达最大,之后逐渐衰减。该乳化液可以很好地降低胃肠环境对乳酸杆菌的伤害。

Preparation of new attapulgite probiotic emulsion

Attapulgite clay, soy protein, vegetable oil and Tween 80 were used to prepare Lactobacillus emulsion. By comparing the encapsulation rate, the results of the water-in-oil type were better than those of the oil-in-water type. The best encapsulation rate of the water-in-oil emulsion was 92.31% by the optimal orthogonal experiments. The viable cell was 5.171×10¹⁰ CFU/mL, and the emulsification was 2.176. The cells in the emulsion in the artificial simulation gastric juice declined with the time increasing, then constantly released in the artificial simulation intestinal juice, and the cells reached the maximum at the sixth hours, then gradually decay. The emulsion can reduce the damage of gastrointestinal environment for Lactobacillus.