Determination of metals in fish livers by flameless atomic absorption spectroscopy

The samples are digested in nitric acid and evaporated to near dryness. Hydrogen peroxide is then added for complete oxidation of fat residues. Determination of metals is performed by flameless AAS with graphite furnace and results evaluated by the method of standard addition. The method is suitable for the determination of some non-volatile metals in fish livers in which the amount of available sample is limited; Pb, Cd, Cu and Zn have been determined in such samples.