Aggregation mechanism of bovine serum albumin based on collision factor by the population balance conservation law

This paper discusses a novel approach for exploring the aggregation mechanism of bovine serum albumin using collision factor. The population balance equation consisting of aggregation term was developed and solved by the moment method. Different experiments were implemented to account for effective parameters on protein aggregation and to measure variations in average size of aggregates formed in a time interval. This was done by taking pictures with a CCD camera on a sterio microscope. The pictures were exported to image processing software to analyze average number and size of aggregates. The collision factor appearing in population balance equation was optimized and declared as a global term.