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地衣芽孢杆菌固态发酵代谢产物分析
引用本文:王和玉,杨帆,林琳,王莉,杨代永,吕云怀,季克良.地衣芽孢杆菌固态发酵代谢产物分析[J].酿酒科技,2011(9):32-34,37.
作者姓名:王和玉  杨帆  林琳  王莉  杨代永  吕云怀  季克良
作者单位:贵州茅台酒股份有限公司技术中心,贵州仁怀,564501
摘    要:从茅台酒生产环境中分离纯化得到1株细菌MTDB-02,经16S rDNA测序比对,最终将MTDB-02定性为地衣芽孢杆菌。将该菌在制曲工艺、堆积工艺以及兼性条件下分别进行纯种固态发酵,利用GC-MS对其代谢物进行检测解析。结果表明,该菌纯种固态发酵能代谢产生70多种物质。这些物质中有多种是食品中的风味物质和风味前体物质,亦是茅台酒呈香呈味物质中的一个组成部分。

关 键 词:微生物  地衣芽孢杆菌  固态发酵  代谢产物  茅台酒风味

Analysis of the Metabolites of Bacillus licheniformis by Solid Fermentation
WANG Heyu,YANG Fan,LIN Lin,WANG Li,YANG Daiyong,LU Yunhuai , JI Keliang.Analysis of the Metabolites of Bacillus licheniformis by Solid Fermentation[J].Liquor-making Science & Technology,2011(9):32-34,37.
Authors:WANG Heyu  YANG Fan  LIN Lin  WANG Li  YANG Daiyong  LU Yunhuai  JI Keliang
Affiliation:WANG Heyu,YANG Fan,LIN Lin,WANG Li,YANG Daiyong,LU Yunhuai and JI Keliang(Technology Center of Moutai Co.Ltd,Renhuai,Guizhou 564501,China)
Abstract:MTDB-02,was easily isolated from the production environment of Moutai Liquor.MTDB-02 was identified as Bacillus licheniformis by 16S rDNA identification.Then pure solid fermentation of MTDB-02 was carried out under starter-making conditions,stacking conditions,and facultative conditions respectively and GC-MS was applied to anlayze its metabolites.The results showed that MTDB-02 could produce more than 70 metabolites by pure solid fermentation.Most of these metabolites were flavoring compounds and flavoring...
Keywords:microbe  Bacillus licheniformis  solid fementation  metabolites  Maotai flavor  
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