| 白腐菌预处理木屑产漆酶条件的优化及其糖化评价 |
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| 引用本文： | 罗少华,程金花,龚大春.白腐菌预处理木屑产漆酶条件的优化及其糖化评价[J].酿酒科技,2011(10):17-20. |
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| 作者姓名： | 罗少华 程金花 龚大春 |
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| 作者单位： | 三峡大学化学与生命科学学院,湖北宜昌,443002 |
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| 基金项目： | 湖北省教育厅重点项目(CXY2009B008); 国家自然基金项目(21076114) |
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| 摘 要： | 对白腐菌固态发酵预处理木屑的工艺条件进行了优化,并对经过白腐菌预处理木屑的糖化效果进行了评价。结果表明,白腐菌固态发酵产漆酶的工艺条件为:1 kg木屑中需添加麦麸120 g,氮酒石酸铵60 g,料水比0.28,微量元素的用量为7 mL/L,培养时间为3 d,白腐菌产漆酶的酶活最高。在最佳工艺条件下,经白腐菌生物预处理的木屑的糖化率可以提高1倍,生物预处理木屑具有一定的应用前景。
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| 关 键 词： | 微生物 白腐菌 漆酶 优化 预处理 糖化 |
Optimization of Laccase-producing Conditions of Wood Chips Pretreated by White-rot fungi and Evaluation of Its Saccharification |
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| LUO Shaohua,CHENG Jinhua , GONG Dachun.Optimization of Laccase-producing Conditions of Wood Chips Pretreated by White-rot fungi and Evaluation of Its Saccharification[J].Liquor-making Science & Technology,2011(10):17-20. |
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| Authors: | LUO Shaohua CHENG Jinhua GONG Dachun |
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| Affiliation: | LUO Shaohua,CHENG Jinhua and GONG Dachun (College of Chemistry and Life Science,Three Gorges University,Yichang,Hubei 443002,China) |
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| Abstract: | The technical conditions of wood chips pretreated by white-rot fungi solid fermentation had been optimized as follows: 120g wheat bran added in per kg wood chips as carbon source and 60 g tartaric acid ammonium added as nitrogen source,the ratio of raw materials and water was 0.28,the use level of trace elements was 7 ml/L,and culture time was 3 d.Besides,the saccharifying effects of wood chips were evaluated.Under the above conditions,the activity of the produced laccase was the highest and the saccharifyi... |
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| Keywords: | microbe white-rot fungi laccase optimization pretreatment saccharification |
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