| 脱氧雪腐镰刀菌烯醇的快速分离纯化及其抗体制备 |
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| 引用本文： | 洪淑娟,傅坚英,曹娟,喻子牛,张吉斌.脱氧雪腐镰刀菌烯醇的快速分离纯化及其抗体制备[J].化学与生物工程,2013,30(9):18-22. |
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| 作者姓名： | 洪淑娟 傅坚英 曹娟 喻子牛 张吉斌 |
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| 作者单位： | 华中农业大学农业微生物学国家重点实验室,湖北武汉,430070 |
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| 基金项目： | 国家973计划资助项目 |
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| 摘 要： | 采用高速逆流色谱法结合高效液相色谱法从禾谷镰刀菌固体发酵产物中分离纯化脱氧雪腐镰刀菌烯醇(DON)。先用高速逆流色谱在以乙酸乙酯-水(1∶1,体积比)为两相溶剂系统、温度为25℃、主机转速为890r·min-1、流速为1.0mL·min-1、检测波长为254nm的条件下进行分离,再用高效液相色谱进一步分离纯化,最终从300g大米发酵培养物中得到了8mg DON,纯度为97.7%。将纯化的DON用于DON人工抗原制备,基质辅助激光解吸附电离串联飞行时间质谱分析表明,人工抗原制备成功;免疫动物后,研制出了高效价的多克隆抗体。本研究建立的色谱方法操作简单,分离效果较好,纯化的DON可以用于抗体的制备。
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| 关 键 词： | 脱氧雪腐镰刀菌烯醇 禾谷镰刀菌 高速逆流色谱 高效液相色谱 DON人工抗原 多克隆抗体 |
Rapid Separation and Purification of Deoxynivalenol by High-Speed Counter-Current Chromatography Coupled with High Performance Liquid Chromatography and Its Antibody Production |
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| HONG Shu-juan
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FU Jian-ying
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CAO Juan
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YU Zi-niu
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ZHANG Ji-bin.Rapid Separation and Purification of Deoxynivalenol by High-Speed Counter-Current Chromatography Coupled with High Performance Liquid Chromatography and Its Antibody Production[J].Chemistry & Bioengineering,2013,30(9):18-22. |
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| Authors: | HONG Shu-juan FU Jian-ying CAO Juan YU Zi-niu ZHANG Ji-bin |
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| Affiliation: | HONG Shu-juan;FU Jian-ying;CAO Juan;YU Zi-niu;ZHANG Ji-bin;State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University; |
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| Abstract: | |
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| Keywords: | deoxynivalenol Fusarium graminearum high-speed counter-current chromatography high performance liquid chromatography DON artificial antigen polyclonal antibody |
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