Antioxidant activity of twenty wild Spanish Thymus mastichina L. populations and its relation with their chemical composition |
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Authors: | Teresa Delgado Pilar Marinero M Carmen Asensio-S-Manzanera Carmen Asensio Baudilio Herrero José Alberto Pereira Elsa Ramalhosa |
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Affiliation: | 1. CIMO, School of Agriculture, Polytechnic Institute of Bragança, Campus de Santa Apolónia, Apartado 1172, 5301-854 Bragança, Portugal;2. Instituto Tecnológico Agrario de Castilla y León, Ctra. de Burgos Km.119, 47071 Valladolid, Spain;3. Universidad de Valladolid, Escuela Técnica Superior de Ingenierías Agrarias – Campus “La Yutera”, Avda. Madrid 44, 34004 Palencia, Spain |
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Abstract: | The antioxidant activity and chemical composition of essential oils and methanolic extracts of twenty Spanish Thymus mastichina L. populations were studied. Both essential oils and methanolic extracts possessed antioxidant properties. However, the total phenol contents of the methanolic extracts varied between 2.90 and 9.15 mg GAE/gextract and the EC25 values of DPPH free radical scavenging activity between 0.90 and 3.45 mg/mL for the methanolic extracts and 78–241 mg/mL for essential oils, these showing low antioxidant potential. Actually, in essential oils the main compound determined was the 1,8-cineole (56.8–69.6%), whereas thymol, γ-terpinene, terpinolene and geraniol (species with considerable DPPH scavenging activity) were observed in low amounts. Concerning methanolic extracts, rosmarinic acid was the most abundant polyphenol (1.70–9.85 mg/g), followed by methoxysalicylic acid, apigenin, kaempferol and luteolin. |
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Keywords: | Thymus mastichina L Methanolic extracts Essential oils Antioxidant activity Chemical composition |
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