| Abstract: | Protein-protein interactions are a vital component in both the function and regulation of virtually all biological processes. In the yeast two-hybrid system, one of the most commonly-used methods for studying protein-protein interactions, protein-protein interactions are detected by expression of a reporter gene driven by the interaction between bait and prey proteins. We report here the development of an automated system, the MegaMate, for carrying out high throughput (over 100,000 clones per day) yeast two-hybrid screens with full data tracking. |
