SOD—GC脂质体的制备及包封率的测定

半乳糖神经酰胺（Galactosyl ceramide，简称GC）是一种制备脂质体的新材料，性质稳定，具独特的护肤作用，以GC为主要膜材，用乙醇注入法制备包含有超氧化物歧化酶的脂质体（SOD-GC脂质体）；用SOD蛋白含量法和SOD活性法测定包封率，经正交法优化选择，最佳制备条件为膜脂量1.0mg/mL、SOD量0.1mg/mL，缓冲液pH5.8，此时包土率可达35.68%，两种包封率测定方法结果相近，可以相互参照，这种SOD-GC脂质体有望在护肝肤品和皮肤外用药物治疗等方面应用。

The Preparation and Emcapsulation Effciency Determination of SOD-GC Liposomes

Using galctosyl ceramide (GC) as the main membrane material, SOD - containing liposomes (SOD - GC liposomes) were prepared by alcohol injection method. The optimum preparation condition was screened by orthogonal design, the condition is:SOD concentraion = 0.1 mg/mL, lipids concentration = 1.0 mg/mL, PBS pH = 5.8. Under this condition, the encapsulation efficency is 35.68 %. The encapsulation efficency was calculated by detecting SOD protein mass or SOD activity, the two kinds of results were similar and could be crossed reference. SODGC liposomes can be applied to make skin protection product and deliver drug.