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啤酒中浑浊敏感蛋白的分离与鉴定
引用本文:王建,王德良,张丽叶,贾娟. 啤酒中浑浊敏感蛋白的分离与鉴定[J]. 酿酒, 2007, 34(2): 106-108
作者姓名:王建  王德良  张丽叶  贾娟
作者单位:1. 北京化工大学生命科学与技术学院,北京,100029
2. 中国食品发酵工业研究院,北京,100027
3. 新疆农业大学食品科学学院,乌鲁木齐,830054
摘    要:啤酒非生物稳定性是影响啤酒质量的重要指标之一,非生物浑浊主要是啤酒中的浑浊蛋白质与多酚结合体组成的,浑浊敏感蛋白质主要来自大麦醇溶蛋白,是一种富含脯氨酸的蛋白质.主要阐述了浑浊敏感蛋白质的分离,以及利用蛋白免疫印迹法(Immunobloting)鉴定啤酒中的浑浊敏感蛋白质,此外还研究了硅胶对啤酒的浑浊蛋白质的影响.

关 键 词:浑浊敏感蛋白质  硅胶吸附蛋白质  免疫印迹  啤酒质量  浑浊敏感蛋白  分离与鉴定  Beer  Protein  Identification  硅胶  研究  免疫印迹法  利用  阐述  富含脯氨酸  大麦醇溶蛋白  体组成  结合  多酚  蛋白质  非生物浑浊  指标  影响
文章编号:1002-8110(2007)02-0106-03
收稿时间:2007-01-25
修稿时间:2007-01-25

Isolation and Identification of Haze-Active Protein in Beer
WANG Jian,WANG De-liang,ZHANG Li-ye,JIA Juan. Isolation and Identification of Haze-Active Protein in Beer[J]. Liquor Making, 2007, 34(2): 106-108
Authors:WANG Jian  WANG De-liang  ZHANG Li-ye  JIA Juan
Affiliation:1 .College of Life Science and Technology,Beijing university of Chemical Technology, Beijing,100029,China;2.China National Institute of Food and Fermentation Industries, Beijing, 100027,China; 3.College of Food Science, Xinjiang Agriculture University, Urumqi, 830052, China
Abstract:Non -biology stability of beer is one of the crucial indexes which have impact on the quality of beer. Primarily, non-biology haze is composed of the complex of haze protein and polyphenol. Haze-active protein derives from horderu protein, which is affluent with proline. This article specialized in the separation of haze-active protein, as well as identifying haze-active protein in beer by the utilization of immunobioting. Besides, it also has studied the influence of beer haze -active protein by silica gel.
Keywords:Haze-active protein   Silica eluent protein   Immunobloting
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