| 高效液相色谱法检测酸奶中的唾液酸 |
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| 引用本文： | 徐柳柳,陈丹,马凡提,舒敏,向丽,李博玲,汪锋,李翔宇.高效液相色谱法检测酸奶中的唾液酸[J].中国食品添加剂,2020(5):107-112. |
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| 作者姓名： | 徐柳柳 陈丹 马凡提 舒敏 向丽 李博玲 汪锋 李翔宇 |
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| 作者单位： | 嘉必优生物技术（武汉）股份有限公司;湖北省营养化学品生物合成工程技术研究中心;中国科学院合肥物质科学研究所;中国科学技术大学;武汉工程大学 |
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| 摘 要： | 建立了高效液相色谱-荧光检测器(HPLC-FLD)测定酸奶中唾液酸含量的分析方法。该方法为:使用1moL/L甲酸80℃下水解样品2h,以4,5-亚甲二氧基-1,2-邻苯二铵盐(DMB)为衍生化试剂,在80℃下衍生50min后,利用Zorbax SB-Aq色谱柱对衍生化产物进行分离,荧光检测器检测,外标法定量。该方法唾液酸检出限为0.2mg/L,线性范围0.5~10mg/L之间,线性良好,相关系数为0.9998,加标样品的回收率为88.41%~112.08%之间,相对标准偏差(n=6)为4.73%~10.01%之间。说明该方法检测结果准确,满足检测需求,可用于不同奶制品中唾液酸检测。
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| 关 键 词： | 唾液酸 酸奶 高效液相色谱 |
Determination of sialic acid in yogurt by high performance liquid chromatography |
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| XU Liu-liu,CHEN Dan,MA Fan-ti,SHU Min,XIANG Li,LI Bo-ling,WANG Feng,LI Xiang-yu.Determination of sialic acid in yogurt by high performance liquid chromatography[J].China Food Additives,2020(5):107-112. |
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| Authors: | XU Liu-liu CHEN Dan MA Fan-ti SHU Min XIANG Li LI Bo-ling WANG Feng LI Xiang-yu |
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| Affiliation: | (CABIO Bioengineering(Wuhan)Co.,Ltd.,Wuhan 430073;Hubei Province Chemicals Biosynthetic Engineering Technology Research Center,Wuhan 430223;Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031;University of Science and Technology of China,Hefei 230026;Wuhan Institute of Technology,Wuhan 430073) |
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| Abstract: | A method was developed to determine the concentration of sialic acid in yoghurt by HPLC-FLD.Sialic acid was released by 1 mol/L Formic acid hydrolysis for 2 hours under the temperature of 80℃and then derivatization with 4,5-methylenedioxy-1,2-phenylenediamine dihydrochloride at 80℃for 50 min in the dark.The separation and detection of sialic acid derivatives was obtained on Zorbax SB-Aq column.The results indicated that limits of detection was 0.2 mg/L,the linear range was from 0.5 to 10 mg/l with a good linearity(r=0.9998),the spike recovery rate of sialic acid was from 88.41%to 112.08%,and the relative standard deviation was from 4.73%to 10.01%.It shows that the method is accurate and can be applied in the determination of sialic acid in different kinds of dairy products. |
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| Keywords: | sialic acid yoghurt high performance liquid chromatography(HPLC) |
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