超声-微波协同法提取荔枝皮原花青素及其高聚体组成分析

为提高荔枝皮中原花青素的得率和进一步阐明其高聚体组成,本文以糯米糍荔枝为原料,采用超声-微波协同法提取荔枝皮原花青素(litchi pericarp proanthocyanidins,LPC),并运用响应面法优化其工艺条件,将分离所得的高聚原花青素(litchi pericarp polymeric proanthocyanidins,LPPC)用薄层层析色谱(TLC)初步定性,然后运用基质辅助激光解析电离-飞行时间质谱(MALDI-TOF-MS)分析其组成。结果表明,最佳提取工艺条件为:微波功率307W,时间17min,温度46℃,料液比1∶31g/mL,额定超声频率50kHz,优化后得率为8.10%;LPPC以四至六聚体为主,检测到最高聚体为十二聚体,没食子酸酰化程度低且富含A型键,由检测结果可见,各聚合度LPPC至少含有一种含A型键的结构。

Ultrasonic-microwave synergistic extraction of proanthocyanidins from litchi pericarp and characterization of polymeric proathocyanidins by MALDI-TOF-MS

To optimize the conditions for extracting proanthocyanidins from litchi pericarp(LPC)by the ultrasonicmicrowave synergistic method,the response surface methodology was employed and the MALDI-TOF-MS was used to characterize the polymeric proanthocyanidins from nuomici litchi pericarp(LPPC).The results showed that the optimal conditions for extraction process were as followed:microwave power of 307W,extraction time of 17min,extraction temperature of 46℃,material-liquid ratio of 1∶31g/mL,and the optimized yield was 8.10%.LPPC was mainly composed of tetramers and hexamers.The gallic acid had a low degree of acylation and was rich in A-type bonds.The highest degree of polymerization was detected as dodecamer and there was at least one structure containing A-type bonds which was detected in each polymer.