Production of Branched Cyclodextrins by Reverse Reaction of Microbial Debranching Enzymes

Formation of maltosyl cyclodextrins from mixtures of maltose and cyclodextrins by reverse reactions of Flavobacterium isoamylase and Klebsiella pullulanase was experimented and it was found that Klebsiella pullulanase produced 50.4mg maltosyl α-cyclodextrin, 35.0mg maltosyl β-cyclodextrin and 55.4mg maltosyl γ-cyclodextrin per ml of reaction mixture, whereas Flavobacterium isoamylase did not form maltosyl cyclodextrins. Optimum conditions for formation of maltosyl β-cyclodextrin by Klebsiella pullulanase were pH 4, 50°C reaction temperature and proportion of substrate 100mg β-cyclodextrin/600mg maltose per ml.