| 治疗性乙型肝炎病毒载体疫苗的构建 |
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| 引用本文： | 陈宇,杨小松,李惟,孔维.治疗性乙型肝炎病毒载体疫苗的构建[J].粉末涂料与涂装,2006,19(5):474-476. |
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| 作者姓名： | 陈宇 杨小松 李惟 孔维 |
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| 作者单位： | 吉林大学生命科学学院疫苗研究中心 长春130012 |
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| 摘 要： | 目的研制治疗慢性乙型肝炎的重组修饰的Ankara痘病毒(MVA)载体疫苗。方法将HBV的表面抗原基因克隆到MVA病毒的重组载体pSC11上,重组质粒与空MVA病毒共转染鸡胚成纤维细胞(CEF),进行同源重组。经过10轮筛选,挑选出阳性的重组MVA病毒。经PCR鉴定,感染不同细胞系,确定病毒表达量。结果获得目的重组MVA病毒,ELISA检测到表面抗原在不同细胞系中的表达。结论已成功地构建了表达HBV表面抗原的重组MVA病毒。
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| 关 键 词： | 乙型肝炎 修饰的Ankara痘病毒 治疗性疫苗 |
| 收稿时间： | 2005-12-22 |
| 修稿时间： | 2005年12月22 |
Construction of Recombinant Virus As A Therapeutic Hepatitis B Vaccine |
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| CHEN Yu, YANG Xiao-song, LI Wei, et al.Construction of Recombinant Virus As A Therapeutic Hepatitis B Vaccine[J].Chinese Journal of Biologicals,2006,19(5):474-476. |
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| Authors: | CHEN Yu YANG Xiao-song LI Wei |
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| Affiliation: | Vaccine Research Center, College of Life Science, Jilin University, Changchun 130012, China |
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| Abstract: | Objective To prepare the recombinant modified vaccinia Ankara(MVA) virus expressing HBsAg as a therapeutic vaccine for chronic hepatitis B.Methods Clone HBsAg gene to the shuttle vector pSC11,and co-transfect chick embryo fibroblast(CEF) with the constructed recombinant plasmid and empty MVA virus for homologous recombination.The positive clones were screened,identified by PCR and used for the infection of three different cell lines to determine the expression of HBsAg.Results The HBsAg gene fragment at a length of 681 bp was amplified from the constructed recombinant MVA virus.ELISA proved the expression of HBsAg in all the three cell lines.Conclusion The recombinant MVA virus for expression of HBsAg was successfully constructed. |
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| Keywords: | Hepatitis B Modified vaccinia Ankara(MVA) Therapeutic vaccine |
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