The Helsinki Heart Study: an 8.5-year safety and mortality follow-up

MT-PVLT-10 transgenic mice express the large T-antigen of polyomavirus under the control of the mouse metallothionein-1 promoter. The males of this transgenic line developed testicular tumor and seminal vesicle engorgement at advanced ages. A novel partial cDNA was identified which hybridized to a 2.6 kilobase mRNA. The expression of this mRNA increased approximately two- to fifteen-fold in immortalized cell lines derived from testicular tumors as compared to similar cell lines derived from pre-adenomatous testes. The in vivo pattern of expression for this cDNA as well as its expression in various primary cultures and established cell lines derived from testis of MT-PVLT-10 mice is presented. Overlapping cDNA clones from liver, testes, and brain cDNA libraries containing the entire coding region for this novel cDNA have been isolated and sequenced. The coding region of this gene comprises 1179 nucleotides and predicts a polypeptide of 393 amino acids (calculated molecular mass 44,318). Motif analysis of the amino acid sequence has revealed that it contains several hydrophobic alpha-helices characteristic of transmembrane proteins.