Enhancing thermophilic dark fermentative hydrogen production at high glucose concentrations via bioaugmentation with Thermotoga neapolitana |
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| Affiliation: | 1. Tampere University, Faculty of Engineering and Natural Sciences, P.O. Box 541, FI-33104, Tampere, Finland;2. Department of Civil, Architectural and Environmental Engineering, University of Napoli Federico II, Via Claudio, 21, 80125, Napoli, Italy;3. INRAE, Univ Montpellier, LBE, Narbonne, France;1. Key Laboratory of Poyang Lake Environment and Resource Utilization, Ministry of Education, School of Resources Environmental & Chemical Engineering, Nanchang University, Nanchang, 330031, China;2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China;3. Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing, 100048, China;1. Department of Environmental Engineering, Daegu University, Gyeongbuk 38453, Republic of Korea;2. Department of Chemical Engineering, Kyonggi University, Suwon 16227, Republic of Korea;3. School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea;1. Advanced Technology Development Centre, Indian Institute of Technology, Kharagpur 721302, India;2. Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India;3. Department of Chemical Engineering, Indian Institute of Technology, Kharagpur 721302, India;1. Dokuz Eylul University, Department of Environmental Engineering, Izmir, Turkey;2. Dokuz Eylul University, The Graduate School of Natural and Applied Sciences, Department of Biotechnology, Izmir, Turkey;1. Sustainable Management of Natural Resources and Environment Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam;2. Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea;3. Civil, Environmental and Architectural Engineering, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-714, South Korea;4. IT Convergence Materials R&D Group, Korea Institute of Industrial Technology, Chungnam 330-825, South Korea |
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| Abstract: | The aim of the present study was to investigate the effect of gradually increasing glucose concentrations (from 5.6 to 111 mmol L−1) on the fermentative H2 production with and without bioaugmentation. A stirred tank reactor (STR) was operated at 70 °C and inoculated with a hyperthermophilic mixed culture or a hyperthermophilic mixed culture bioaugmented with Thermotoga neapolitana. With both the unaugmented (control) and augmented cultures, the H2 production rate was improved when the initial glucose concentration was increased. In contrast, the highest H2 yield (1.68 mol H2 mol−1 glucose consumed) was obtained with the augmented culture at the lowest glucose concentration of 5.6 mmol L−1 and was 37.5% higher than that obtained with the unaugmented culture at the same feed glucose concentration. Overall, H2 production rates and yields were higher in the bioaugmented cultures than in the unaugmented cultures whatever the glucose concentration. Quantitative polymerase chain reaction targeting T. neapolitana hydA gene and MiSeq sequencing proved that Thermotoga was not only present in the augmented cultures but also the most abundant at the highest glucose concentrations. |
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| Keywords: | Biohydrogen Dark fermentation Metabolic pathways Microbial dynamics |
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