水痘-带状疱疹病毒gE糖蛋白基因扩增法鉴别水痘减毒活疫苗

目的利用水痘-带状疱疹病毒(VZV)gE糖蛋白基因鉴别水痘减毒活疫苗。方法以提取的病毒DNA为模板,PCR扩增gE糖蛋白基因。经琼脂糖凝胶电泳鉴定。结果水痘减毒活疫苗Oka株VZV(病毒滴度≥2.0lgPFU/ml)通过琼脂糖电泳均可见特异性的条带。使用同样的方法检测麻疹减毒活疫苗、麻疹-腮腺炎联合疫苗、风疹减毒活疫苗以及Ⅰ、Ⅱ型单纯疱疹病毒(病毒滴度≥4.2lgCCID50/ml),结果均为阴性。结论gE糖蛋白基因可以作为VZV的特异性指标,进行病毒鉴别试验。

Identification of Live Attenuated Varicella Vaccine by Amplification of VZV gE Glycoprotein Gene

Objective To identify live attenuated varicella vaccine by the amplification of varicella-zoster virus(VZV) gE glycoprotein gene.Methods Extract viral DNA from the samples of live attenuated varicella vaccine,amplify by PCR and identify by agarose gel electrophoresis.Results The amplified gE genes from live attenuated varicella vaccine prepared with VZV Oka strain at a titer of not less than 2.0 lgPFU/ml showed specific bands on agarose gel electrophoretic profile.However,no VZV gE gene was amplified from the viral DNA of control vaccines,such as live attenuated measles vaccine,measle-mumps combined vaccine and rubella vaccine as well as herpes simplex virus types Ⅰ and Ⅱ,each at a titer of not less than 4.2 lgCCID 50/ml.Conclusion gE glycoprotein gene might be used as a specific indicator for the identification of VZV.