碳化二亚胺/羟基丁二酰亚胺交联改性胶原蛋白纤维制备及其性能

为解决由湿法纺丝工艺制备的牛肌腱胶原蛋白纤维力学性能差、遇水易溶解等问题,采用1-乙基-3-(3-二甲基丙基)碳化二亚胺盐酸盐/N-羟基丁二酰亚胺(EDC/NHS)对胶原蛋白纤维进行交联改性。探究了原位交联方式的最优交联时间和交联剂质量分数,并着重对比了原位交联与交联浴交联2种交联方式对胶原蛋白纤维性能的影响。结果表明:原位交联可显著改善胶原蛋白纤维的性能,最优的交联时间和交联剂质量分数分别为11 h、15%,此时纤维断裂强度可达1.44 cN/dtex左右,较纯胶原蛋白纤维提高了35.8%,较交联浴交联纤维提高了19.0%;与主要发生在纤维表面的交联浴交联相比,原位交联能够使纤维内部的微纤结构更加致密,性能得到更为显著的提升,由原位交联制备的胶原蛋白纤维性能优于交联浴交联制备的纤维。

Carbodiimide/hydroxysuccinimide crosslinking modification and properties of collagen fibers

In order to deal with problems in poor mechanical properties and water-dissolving,a collagen fiber prepared from bovine tendon type I collagen via wet spinning method process was modified by 1-ethyl-3-(3-dimethylpropys carbodiimide/N-hydroxysuccinimide)(EDC/NHS)crosslinking.The optimal reaction time and crosslinking concentration of EDC/NHS in the in-situ crosslinking mode were explored,and the effects of two crosslinking methods of in-situ crosslinking and bath crosslinking on the properties of collagen fibers were compared.The results show that in-situ crosslinking can significantly improve the performance of collagen fibers.The optimum crosslinking time and crosslinker concentration of in-situ crosslinking are 11 h and 15%,respectively.In this situation,the fiber breaking strength reached about 1.44 cN/dtex,which is 35.8%higher than that of the pure collagen fiber and 19.0%higher than that of bath crosslinked fiber.Compared with crosslinking baths that the crosslinking reaction mainly occur on the fiber surface,and in-situ crosslinking makes the microfibrous structure inside the fiber denser and the performance is improved more obviously.Furthermore,the performance of in-situ crosslinked collagen fibers is better than that of bath crosslinked collagen fibers.