光交联丝素蛋白水凝胶的蓝光引发体系

针对再生丝素蛋白水凝胶生物活性、凝胶效率及凝胶强度三者难以兼具的问题,用樟脑醌(CQ)、核黄素磷酸钠(FMN)、曙红Y(EY)及姜黄素4种生物相容的光敏剂,分别与二芳基六氟磷酸碘鎓盐(DPI)构成丝素蛋白水凝胶的高效蓝光交联引发体系。借助紫外分光光度计和光差示扫描量热法研究了4种光引发体系的光谱吸收特性和蓝光引发效率,采用光流变系统研究了光交联丝素蛋白的凝胶化行为。结果表明:CQ/DPI、EY/DPI体系均可作为自由基聚合以及丝素蛋白光交联反应的良好蓝光引发体系,该蓝光引发体系诱导丝素蛋白酪氨酸残基自由基间的偶合反应是丝素蛋白光交联反应的主要机制;以EY/DPI体系引发低浓度丝素蛋白光交联反应可在短时间(10 min)内实现较高强度丝素蛋白水凝胶的制备。

Blue light initiated photocrosslinking of silk fibroin hydrogel

A rapid fabrication method for high-strength silk fibroin hydrogel using blue light photocrosslinking was developed. To achieve this, different photoinitiators (camphorquinone, riboflavin sodium phosphate, curcumin and Eosin Y) together with diphenyl iodonium hexafluorophosphate (DPI) were composed to five efficient blue light photoinitiation systems for the photo-crosslinking of silk fibroin. The spectral absorption characteristics and the photopolymerization efficiency of five photoinitiators were investigated by UV-vis spectrophotometer and photo-DSC. The crosslinking performance of silk fibroin hydrogel was investigated by photo-rheology. The UV-vis spectrum indicates five photoinitiators can be served as blue light photoinitiators and photocrosslinking of silk fibroin can be attributed to the dityrosine by coupling of tyrosine residues. Camphorquinone and Eosin Y show a higher photopolymerization efficiency than other photoinitiators. With such photoinitiation systems, silk fibroin hydrogel is prepared within 10 min in this research.