An integrated microfabricated device for dual microdialysis and on-line ESI-ion trap mass spectrometry for analysis of complex biological samples |
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Authors: | Xiang F Lin Y Wen J Matson D W Smith R D |
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Affiliation: | Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA. |
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Abstract: | A microfabricated dual-microdialysis device in a single integrated microfabricated platform was constructed using laser micromachining techniques for the rapid fractionation and cleanup of complex biological samples. On-line dual microdialysis and ESI-MS of biological samples was demonstrated using an ion trap mass spectrometer. The mass spectra obtained demonstrated the efficiency of dual microdialysis for removing both high-molecular-weight and low-molecular-weight species that interfere with effective ESI-MS analysis of target biopolymers. Signal-to-noise ratios were also greatly improved compared to direct sample infusion. In addition to its compactness, negligible dead volume, and robustness, the device can be used at a flow rate of only 200 nL/min, an order of magnitude lower than that obtained previously. This reduced sample consumption and improved sensitivity with ESI-MS. The results suggest the potential for integration of such microfabricated devices with other sample manipulations for the rapid ESI-MS analysis of complex biological samples. |
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