EGCG对高脂饮食诱导的肥胖HFA小鼠脂质代谢的影响

利用高脂饮食诱导人源菌群(human flora-associate,HFA)肥胖小鼠模型,探讨表没食子儿茶素没食子酸酯(EGCG)对肥胖小鼠的降脂功效。无菌昆明(KM)小鼠灌胃健康人粪便悬液构建HFA模型后随机分为两组:高脂组40只和普通组10只,分别喂食高脂饲料和普通饲料,8周后各处死10只,测定小鼠体重、肝重、附睾脂肪垫重量、血糖血脂含量以及肝脏和附睾脂肪垫苏木精-伊红(HE)染色。将获得的肥胖小鼠随机分为三组,即对照组,0.2%EGCG低剂量组和0.8%EGCG高剂量组,喂食普通饲料,8周后全部处死,测定同上指标。结果表明高剂量组小鼠附睾脂肪垫重量、总胆固醇(Tch)和低密度脂蛋白胆固醇(LDL-C)含量均较对照组显著降低,分别降低了48.15%、28.64%和43.16%,同时脂肪细胞大小较对照组也显著减小,除此之外EGCG干预组肝脏病变程度较对照组有明显好转。由此证实,EGCG对肥胖HFA小鼠降脂及肝脏损伤修复有一定的作用。

Effects of EGCG on the Lipid Metabolism in Obese HFA Mice Induced by High-Fat-Diet

The lipid-lowering effects of (-)-epigallocatechin-3-gallate (EGCG) on obese human flora-associate (HFA) mice induced by high fat diet were investigated in this study. The aseptic Kunming (KM) mice were inoculated with healthy human excrement suspension to construct HFA model and randomly divided into two groups: high-fat group (40) and normal group (10) were fed with high-fat diet and normal diet, respectively. After 8 weeks, the mice were sacrificed and the body weight, liver weight, epididymal fat pad weigh, blood glucose, blood lipid and hematoxylin and eosin (HE) staining of liver and epididymal fat pad were measured. The obese mice were randomly divided into three groups: the control group, 0.2% EGCG low-dose group and 0.8% EGCG high-dose group, and fed with normal diet After 8 weeks, the mice were sacrificed and several indices mentioned above were assayed. The results showed that the epidihymal fat pad weight,total cholesterol (Tch) and low density lipoprotein content (LDL-C) in high dose group were significantly decreased by 48.15%, 28.64% and 43.16%, respectively, as compared to the normal group. Furthermore, EGCG treatment also normalized the cellular morphology of the epidihymal fat pad, and alleviated hepatic damage in a dose-dependent manner. These results suggested that EGCG had certain effects on lipid-decreasing and liver injury repair activity in obese HFA mice.