| TLC快速分析多糖的单糖组成 |
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| 引用本文： | 颜军,郭晓强,李晓光,邬晓勇,苟小军. TLC快速分析多糖的单糖组成[J]. 食品科学, 2006, 27(12): 603-607 |
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| 作者姓名： | 颜军 郭晓强 李晓光 邬晓勇 苟小军 |
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| 作者单位： | 成都大学中药化学实验室； |
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| 基金项目： | 四川省中医药管理局资助项目;四川省中医药管理局资助项目 |
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| 摘 要： | 本文采用薄层色谱法分析多糖的单糖组成。从斑点分离度、清晰度、有无拖尾现象及展层时间等方面综合评价展层效果。通过实验确定了适合的展开剂为展开系统(乙酸乙脂:吡啶:无水乙醇:水=8:1:1:2)、合适的显色剂为苯胺-二苯胺磷酸显色剂。通过此体系确定了单糖的Rf值,可用于快速分析水解多糖的单糖组分。
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| 关 键 词： | 多糖 单糖组成 薄层色谱 |
| 文章编号： | 1002-6630（2006）12-0603-05 |
| 收稿时间： | 2006-07-26 |
| 修稿时间： | 2006-07-26 |
TLC to Fleetly Analyze Monosaccharide Composition of Polysaccharide |
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| YAN Jun,GUO Xiao-qiang,LI Xiao-guang,WU Xiao-yong,GOU Xiao-jun. TLC to Fleetly Analyze Monosaccharide Composition of Polysaccharide[J]. Food Science, 2006, 27(12): 603-607 |
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| Authors: | YAN Jun GUO Xiao-qiang LI Xiao-guang WU Xiao-yong GOU Xiao-jun |
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| Affiliation: | Laboratory of Traditional Chinese Medicine, Chengdu University, Chengdu 610106, China |
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| Abstract: | Applying TLC to analyze monosaccharide composition of polysaccharides was stuided. A mixed solution composed of ethylacetate, pyridine, alcohol and water in a ratio of VC4H8O2:VC5H 5N:VC2H6O:VH2O = 8:1:1:2 by volume is used as extending system. A solution containing aniline and diphenylamine dissolved in acetone is used as coloration system. Satisfactory separation is acquired as shown by the thin layer chromatogram. Qualitative results can be obtained in accordance with the Rf value of the standard spots. Distinct figures indicating the monosaccharide composition of polysaccharides obtained by the modified TLC.The extending system and coloration system can be used in monosaccharide composition analysis. |
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| Keywords: | polysaccharide monosaccharide composition thin-layer chromatography ( TLC) |
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