多糖组分色谱指纹图谱聚类分析可可粉的掺假鉴别

为了建立可可粉掺假检测方法,采用超声辅助提取可可粉中多糖组分,利用硫酸水解及柱前PMP(1-苯基-3-甲基-吡唑啉酮)衍生化高效液相色谱法(HPLC)分析得到可可粉多糖组分的指纹图谱,根据共有特征峰的相对峰面积,应用系统聚类分析(HCA)法进行可可粉掺假鉴别试验。结果表明,掺入15%及以上可可壳的可可粉可以通过此方法予以鉴别。

Adulteration Detection of Cocoa Powder by Clustering Analysis of Polysaccharide Components

In order to establish cocoa adulterating detection methods,polysaccharide contained in cocoa powder was extracted by Ultrasound assisted method,then hydrolyzed with sulfuric acid solution,and finally derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP) to get cocoa polysaccharide fingerprints by high performance liquid chromatography(HPLC).According to the relative peaks areas of the common peaks,adulteration detection of cocoa powder was attempted successfully by system clustering analysis(HCA) method.The results showed that cocoa powder adulterated with more than 15% cocoa shell could be identified by this method.