豆渣可溶性膳食纤维酶法制备工艺及其品质分析

为了获得高得率的豆渣可溶性膳食纤维,以碱处理豆渣制备可溶性膳食纤维后剩余的不溶性残渣为原料,采用纤维素酶对其进行酶解改性。通过单因素试验和响应面优化试验,研究了不同酶解条件对豆渣可溶性膳食纤维得率的影响。结果表明:对豆渣可溶性膳食纤维得率的影响因素依次为加酶量>酶解时间>酶解温度>酶解pH,最佳酶解工艺条件为:加酶量1.80%,酶解时间3.5 h,酶解温度48℃,酶解pH4.8。在此条件下,豆渣可溶性膳食纤维得率可达到7.64%,且其品质符合国家粮食行业标准规定的指标。扫描电镜结果表明,酶法制备的豆渣可溶性膳食纤维的颗粒较小,呈现蜂窝状,有利于其水合特性的提高。

Study on Preparation of Soluble Dietary Fiber from Soybean Residue by Enzymatic Method and Its Quality Analysis

Soluble dietary fiber was obtained from insoluble dietary fiber of soybean residue which was alkali hydrolyzed by treatment with cellulase.Based on the single-factor experiment and the response surface experiment,the relationships between the yield of soluble dietary fiber and enzymolysis factors were studied.Results showed that the effect of four factors on the yield of soluble dietary fiber orderly from high to low was as follows: enzyme dossage,enzymolysis time,enzymolysis temperature,and pH.The optimum enzymolysis conditions were as follows: enzyme dossage 1.80%,enzymolysis time 3.5 h,enzymolysis temperature 48℃,pH 4.8.Under these conditions,the yield of soluble dietary fiber could reach 7.64%,and the quality of soluble dietary fiber was consistent with the quality of the country's grain industry standards.Results from scanning electron microscopy showed that the particle of soluble dietary fiber was smaller and honeycomb which was beneficial to the improvement of its hydration.