EXTRACTION OF PROTEINS FROM BUCKWHEAT BRAN |
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Authors: | MARIA V GROSSMAN CHIGURUPATI S RAO RUI SERGIO F DA SILVA |
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Affiliation: | Departmento de Tecnologia de Alimentos e Medicamentos Universidade Estadual de Londrina Cx. Postal 6001 Londrina, 86100 Parana, Brasil |
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Abstract: | ABSTRACT Proteins from buckwheat bran were extracted by a wet alkaline extraction process, giving a yield of 37.0%of the total nitrogen in the liquid extract, at pH of 6.5. However, the yield could be improved, given a pretreatment of the bran with carbohydrases, thus producing a yield of 56% with cellulase, 58%with pectinase, whereas the increase was minimal with hemicellulase. The maximum yield of 67.5%was obtained by synergistic action of pectinase and hemicellulase, in a pretreatment lasting for 7 h at pH of 3.7. |
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