| Voltammetry and fluorescence studies on the interaction between Eu(phen)_3~(+3)and DNA |
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| 引用本文： | KANG Jingwan,CHEN Jing,LU Xiaoquan,SONG Yuming,ZENG Hongjuan,LIU Hongde,and WANG yongshengDepartment of Chemistry,Northwest Normal University,Lanzhou 730070,China (Received 2002-04-02). Voltammetry and fluorescence studies on the interaction between Eu(phen)_3~(+3)and DNA[J]. 稀有金属(英文版), 2003, 22(2) |
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| 作者姓名： | KANG Jingwan CHEN Jing LU Xiaoquan SONG Yuming ZENG Hongjuan LIU Hongde and WANG yongshengDepartment of Chemistry Northwest Normal University Lanzhou 730070 China (Received 2002-04-02) |
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| 作者单位： | Departnent of Chemistry, Northwest Normal University, Lanzhou 730070, China |
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| 基金项目： | This project is financially supported by the Natural Foundation of Gansu Province (GZ 97-0025)，NWNUKJCXGC-01(No. 001) |
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| 摘 要： | The binding mode of Eu(phen) 3+3 with DNA is studied by fluorescence and voltammetric methods. From the Scatchard graph and the fluorescence quenching phenomenon, it is concluded that the mode of interaction between Eu(phen)3+3 and DNA is intercalation bind by inserting the phen ?-? conjugate surface between the base pairs of the DNA duplex. Voltammetry was used to confirm the results obtained from the fluorescence method, the result from both methods agrees with each other. The binding constant (K) and the binding site size (ns) were calculated from voltammetric data such as the shifts in potential and limiting currents in the process of adding DNA, according to the positive shifts of peak potential. It was considered that the complex of the +2 ion interacted more favorably with the nucleotide bases than that of the +3 ion by hydrophobic interaction.
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Voltammetry and fluorescence studies on the interaction between Eu (phen)33+ and DNA |
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| Kang Jingwan,CHEN Jing,Lu Xiaoquan,SONG Yuming,ZENG Hongjuan,LIU Hongde,WANG yongsheng. Voltammetry and fluorescence studies on the interaction between Eu (phen)33+ and DNA[J]. Rare Metals, 2003, 22(2) |
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| Authors: | Kang Jingwan CHEN Jing Lu Xiaoquan SONG Yuming ZENG Hongjuan LIU Hongde WANG yongsheng |
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| Affiliation: | Departnent of Chemistry, Northwest Normal University, Lanzhou 730070, China |
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| Abstract: | The binding mode of Eu(phen) 3+3 with DNA is studied by fluorescence and voltammetric methods. From the Scatchard graph and the fluorescence quenching phenomenon, it is concluded that the mode of interaction between Eu(phen)3+3 and DNA is intercalation bind by inserting the phen ?-? conjugate surface between the base pairs of the DNA duplex. Voltammetry was used to confirm the results obtained from the fluorescence method, the result from both methods agrees with each other. The binding constant (K) and the binding site size (ns) were calculated from voltammetric data such as the shifts in potential and limiting currents in the process of adding DNA, according to the positive shifts of peak potential. It was considered that the complex of the +2 ion interacted more favorably with the nucleotide bases than that of the +3 ion by hydrophobic interaction. |
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| Keywords: | electroanalysis chemistry voltammetry fluorescence Eu(phen) 3+3 DNA intercalation bind |
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