| 固相萃取-高效液相色谱法测定鸡肉中氯霉素的残留 |
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| 引用本文： | 何方奕,李铁纯,李学程,许可,回瑞华. 固相萃取-高效液相色谱法测定鸡肉中氯霉素的残留[J]. 食品科学, 2006, 27(12): 629-630 |
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| 作者姓名： | 何方奕 李铁纯 李学程 许可 回瑞华 |
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| 作者单位： | 鞍山师范学院化学系； |
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| 摘 要： | 本文采用固相萃取-高效液相色谱法对肉食鸡中氯霉素残留进行检测。样品用乙酸乙酯提取,并用正己烷脱酯,经ENVI-18固相萃取小柱净化后,采用C18色谱柱分离,流动相甲醇:水=35:65(V/V),流速为1ml/min液相色谱分析,紫外检测波长为280nm。在1～200μg/ml范围内,峰面积与氯霉素浓度呈良好的线性关系(r=0.9992),精密度(RSD)小于5%,检测限为0.005μg/g,平均回收率为82.4%。
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| 关 键 词： | 氯霉素(CAP)残留 鸡肉 固相萃取(SPE) 高效液相色谱(HPLC) |
| 文章编号： | 1002-6630（2006）12-0629-02 |
| 收稿时间： | 2006-08-20 |
| 修稿时间： | 2006-08-20 |
Determination of Chloramphenicol Residues in Chichen by SPE-HPLC |
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| HE Fang-yi,LI Tie-chun,LI Xue-cheng,XU Ke,HUI Rui-hua. Determination of Chloramphenicol Residues in Chichen by SPE-HPLC[J]. Food Science, 2006, 27(12): 629-630 |
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| Authors: | HE Fang-yi LI Tie-chun LI Xue-cheng XU Ke HUI Rui-hua |
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| Affiliation: | Department of Chemistry, Anshan Normal University, Anshan 114005, China |
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| Abstract: | The chloramphenicol in chichen was assayed with the method of solid phase extraction and high performance liquid chromatography (HPLC). The chichen sample was extracted by ethylacetate and cleaned by ENVI-18 SPE column. Then the sample was separated by the C18 column with a mobile phase of methanol and water(35:65, V/V), at the rate of 1ml/min and detected at 278nm. The RSD of this method is less than 5%, and the detection limit was 0.005μg/g.The everage recoveries was 82.4%. |
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| Keywords: | chloramphenicol residues chichen solid phase extraction high performance liquid chromatography(HPLC) |
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