薄层色谱法快速分析乳果糖

以硅胶G板为固定相,正丁醇-乙醇-水(体积比5:3:2)为流动相,研究并建立了乳果糖的薄层层析快速分析方法。经过上行展开,苯胺-二苯胺-磷酸显色,根据葡萄糖、半乳糖、果糖、乳糖、乳果糖各斑点的R_f值和各自的斑点颜色定性。运用飞点扫描仪扫描乳果糖斑点的峰面积,并依据建立的标准曲线计算酶反应液中的乳果糖含量。在所选择的实验条件下,乳果糖的检测限为2μg,在2～30μg范围内,其斑点的峰面积与检测量具有一定的正相关性。用定量添加标准品的方法研究薄层扫描的准确度,其相对标准偏差为3.41%。此方法设备简单、操作成本低,可以应用于实验室的初期研究阶段。

Rapid Determination of Lactulose by a Thin-layer Chromatography Method

A thin-layer chromatographic method for lactulose determination was established,in which the mobile phase was butanol-alcohol-water (5:3:2,v/v/v).Lactulose,lactose,glucose,galaetose and fruc- tose were developed by ascending technique on the silica gel G plate,and visualized by aniline—diphenyl- amine—phosphoric acid.The Rf value of each saccharide was different and the color of the spots was charac- terized.The calibration curve was linear in the range of 2—30μg and the detection limit was 2μg.To deter- mine the chromatographic peak areas of lactulose,mottles with thin—layer scanner in the sawtooth scanning mode was described.The detective wavelength was 526 nm.The concentration of laetulose was calculated ac- cording to the calibration curve.Accuracy was certified by external standard method.Relative standard devi- ation (RSD) was 3.41%.This method is simple,inexpensive and do not need large-volume unit.It has been applied to the batching of lactulose,which could provide reference for reaction conditions.