| 硫酸对氨基二乙基苯胺－H_2O_2－HRP分光光度法测定HRP的研究 |
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| 引用本文： | 张书圣,焦奎,刘澄凡,秦峰.硫酸对氨基二乙基苯胺－H_2O_2－HRP分光光度法测定HRP的研究[J].青岛科技大学学报,1996(4). |
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| 作者姓名： | 张书圣 焦奎 刘澄凡 秦峰 |
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| 摘 要： | 提出了硫酸对氨基二乙基苯胺－Ｈ２Ｏ２－ＨＲＰ催化动力学光度法测定ＨＲＰ的新方法。ＨＲＰ催化Ｈ２Ｏ２氧化硫酸对氨基二乙基苯胺生成有色化合物，通过测定此有色化合物在λｍａｘ＝５５２．８ｎｍ处的吸光度值间接测定催化剂ＨＲＰ的含量。在所选定的实验条件下，对ＨＲＰ测定的线性范围为１．０×１０－１０～４．０×１０－９ｇ／ｍＬ，检测限为５．７×１０－１１ｇ／ｍＬ。
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| 关 键 词： | 分光光度法 免疫分析 辣粮过氧化酶 硫酸对氨基二乙基苯胺 |
Determination of Horseradish Peroxidase by Spectrophotometry Based on P-Amino-N,N-Diethylaniline Sulphate-H_2O_2-HRP System |
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| Zhang Shusheng ,Jiao Kui, Liu Chengfan, Qin Feng.Determination of Horseradish Peroxidase by Spectrophotometry Based on P-Amino-N,N-Diethylaniline Sulphate-H_2O_2-HRP System[J].Journal of Qingdao University of Science and Technology:Natutral Science Edition,1996(4). |
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| Authors: | Zhang Shusheng Jiao Kui Liu Chengfan Qin Feng |
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| Affiliation: | Department of Applied Chemistry |
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| Abstract: | A new method for the determination of horseradish peroxidase(HRP) was invotigatedby spectrophotometry. It was bsed on H2O2 oxidate P-amino-N, N-diethylaniline sulphate catalyzedby HRP,so HRP could be detected. The linearity range to detect HRP was 1. 0×10-10￣ 4. 0 ×10-9g/mL,and the detection limit 5. 7 × 10-11 g/mL. |
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| Keywords: | spectrophotometry horseradish peroxidase P-amino- N N-diethylaniline sulphate |
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