Phospholipids inTrypanosoma cruzi: Phosphoinositide composition and turnover |
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Authors: | G Racagni M Garcia de Lema C E Domenech E E Machado de Domenech |
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Affiliation: | (1) Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina |
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Abstract: | The polyphosphoinositides fromTrypanosoma cruzi were isolated by preparative thin-layer chromatography (TLC) and identified. When myo-3H]inositol was present in the culture medium for five days, analyses showed the presence of phosphatidylinositol (PI), lysophosphatidylinositol
(lysoPI), phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2). Short-term incubation with32Pi led to higher percentages of incorporation into phosphatidylethanolamine (PE), lysophosphatidylethanolamine (lysoPE) and
PI compared to the other glycerophospholipids. The phosphoinositides (PI, PIP and PIP2) contained a larger proportion of unsaturated than saturated fatty acids. High proportions of 18∶2 were found in the three
phosphoinositides analyzed, whereas the major saturated fatty acid was 18∶0. Watersoluble inositol phosphates (IP, IP2 and IP3) were also identified. |
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