猴头菌转化银杏叶提取物(EGB)工艺条件的研究

在前期优化培养基的基础上,本实验比较了摇瓶的温度、pH、装液量和发酵罐培养的通气量、转速和培养时间等不同培养条件对猴头菌转化EGB生物活性的影响。结果显示,含有0.5%的EGB培养基中,猴头菌转化EGB温度为25～30℃,初始pH范围为5.5～6.5,此时对非酶糖基化反应的抑制率较高。同时添加10g/LCaCO3可稳定pH,提高发酵液对非酶糖基化反应的抑制率。20L发酵罐转化EGB最佳工艺条件为72h前通气量为8L/min,72h后为4.8L/min,转速为120r/min。

Study on Biotransformantion of Ginkgo biloba L. Extract (EGB) by Hericium erinaceus

The effects of cultivation conditions such as temperature, intial pH, and broth volume on the bioacitivities of the biotransformation products of EGB by Hericium erinaceus in shaking flask were further investigated. Results showed that the suitable culture temperature was 25～30 ℃, the optimal intial pH for biotransformation process was 5.5～6.5 in culture medium containing 0.5% EGB. Furthermore, adding the 10 g/L CaCO3 could also ehance the activity. The kinetics parameters of the biotransformation process were analyzed in fermenter scale. The best fermentation technology of 20 L fermentation tank were as follow: the aeration volume was 8 L/min before 72 h and 4.8 L/min after 72 h and agitation ration was 120r/min.