微胶囊大豆异黄酮有效成分的HPLC含量测定

目的:为微胶囊大豆异黄酮的质量研究建立含量测定方法。方法:采用InertsilODS-3(150mm×4.6mm,5μm)色谱柱,1.5%乙酸-甲醇-乙腈(62:18:20)为流动相,检测波长260nm,柱温40℃,流速1.0ml/min。样品用70%的乙醇超声提取,经0.45μm滤膜过滤后进样。结果:HPLC过程于13min内完成,三种组分的分离度均大于1.5。对三批样品中大豆苷元、黄豆苷元和染料木黄酮作了分析,并与原料作了比较,微囊化前后含量呈比例关系。结论:方法简便、快速、准确,为本品以及仅含大豆异黄酮苷元类的保健食品质量控制提供了较为理想的方法。

HPLC Determination of Effective Components in Soybean Isoflavones Microencapsule

Objective: To establish a method for the determination of effective component in soybean isoflavones microencapsule. Methods: The HPLC system consisted of Inertsil ODS-3 column(150 mm×4.6 mm, 5μm), column temperature at 40℃, with 1.5% acetic acid - methanol-acetonitrile (62:18:20) as mobile phase. Detection wavelength was at 260 nm . Samples were extracted by 70% ethanol with ultrasonic wave, and then filtrated with 0.45μm filtration membrane. Results: The separate rates of three component are all higher than 1.5. One determination was finished within 13 minutes. Three batches of sample were analyzed and compared with the raw material. The assays of daidzein, glycitein and genistein showed that there are ratio relationship before and after microencapsulated. Conclusion: The method is simple, rapid and applicable for the determination of soybean isoflavones microencapsule, and it provides a preferable assay method for the quality control of these health-care foodstuffs containing soybean isoflavones aglycones.