采后高CO2处理对板栗褐变相关因子的影响

以‘燕红’板栗为试材,研究了高CO2处理对板栗褐变相关因子的影响效果。将20kg板栗置于大玻璃瓶中,连续通入40%～50%CO2、3%～5%O2的气体在20℃下分别处理3d和5d。处理结束后,将其放置在常温20℃下于货架期第0、5、10d分别取样,测定板栗褐变相关因子的变化。研究结果表明,高CO2处理3s可有效减轻板栗的褐变程度。高CO2处理的板栗三部分的单宁含量均显著地降低,子叶缝合面和中间层后期差异不显著。总酚含量在子叶表层处理显著低于对照,但在子叶缝合面和中间层刚处理完含量要高于对照,后期差异不显著。处理有效地降低了三部分的PPO活性,且在整个货架期中PPO一直保持一个较低的水平。处理在货架期前5d里,显著地降低了POD活性,后期这种差异消失。处理还有效地降低MDA的生成量,处理3d的MDA含量显著地低于对照,较好地保持了膜系统的完整性。综合各指标考虑,高CO2处理3d控制褐变的效果好于处理5d。

Effects of High CO2 Postharvest Treatment on Browning Relative Aspects of Chinese Chestnut

In this paper, effects of high CO2 postharvest treatment on browning relative aspects of Chinese chestnuts (Castanea mollissima Blume.’Yanhong’) harvested from Huairou, Beijing were studied. 20 kg chestnut of each treatment was put into a big glass jar and treated with gas flow (40%～50% CO2, 3%～5% O2) for 3 and 5 days respectively at 20℃. Changes of browning relative aspects were analyzed at 0, 5, 10 days during shelf-life after treatment. It was showed that high CO2 treatment was a positive way to control browning of Chinese chestnuts. High CO2 treatment could decrease tannic content markedly after treatment, but it was not evidently in central layer cotyledon and interlayer cotyledon in later shelf life. Total phenol was lower than CK in cotyledon ectosexine, but it was higher than CK in central layer cotyledon and interlayer cotyledon when just finished the treatment. It was not evidently in the later shelf life. High CO2 treatment inhibited PPO activity and the activity markedly lower than CK during the whole shelf-life in three parts. It also reduced POD activity, but just in early shelf-life days. High CO2 treatment could reduce MDA content. The treatment of 3 days was preferably kept good integrality of membrane system. Considering all indexes synthetically, high CO2 treatment for 3 days better than 5 days.