| 分枝杆菌降解甾醇发酵生产雄烯二酮的两相系统培养基优化 |
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| 引用本文： | 姜绍通,胡锦艳,杨英,赵俊平,崔福强.分枝杆菌降解甾醇发酵生产雄烯二酮的两相系统培养基优化[J].食品科学,2007,28(11):386-390. |
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| 作者姓名： | 姜绍通 胡锦艳 杨英 赵俊平 崔福强 |
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| 作者单位： | 合肥工业大学生物与食品工程学院； 合肥工业大学生物与食品工程学院 安徽合肥230009； 安徽合肥230009； 安徽合肥230009安徽建筑工业学院环境工程系； 安徽合肥230022； |
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| 基金项目： | 国家科技支撑计划;安徽省自然科学基金 |
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| 摘 要： | 对分枝杆菌Mycobacterium sp.BD696-6降解甾醇发酵生产雄烯二酮(AD)的两相系统培养基进行了优化。在甾醇添加量为0.6%时,培养基组成为有机相葵花油浓度20%、碳源糖蜜6%、氮源NH4NO30.3%、磷酸盐(NH4)2HPO40.08%条件下,AD得率最高,达到78.6%,比原培养基条件下得率提高了17.0%。
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| 关 键 词： | 雄烯二酮 甾醇 两相系统 培养基优化 |
| 文章编号： | 1002-6630（2007）11-0386-05 |
| 修稿时间： | 2007-09-05 |
Medium Optimization of Androst-4-ene-3,17-dione Fermentation from Sterols by Mycobacterium sp. in Two-phase Systems |
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| JIANG Shao-tong,HU Jin-yan,YANG Ying,ZHAO Jun-ping,CUI Fu-qiang.Medium Optimization of Androst-4-ene-3,17-dione Fermentation from Sterols by Mycobacterium sp. in Two-phase Systems[J].Food Science,2007,28(11):386-390. |
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| Authors: | JIANG Shao-tong HU Jin-yan YANG Ying ZHAO Jun-ping CUI Fu-qiang |
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| Affiliation: | 1.School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China; 2.Department of Environment Engineering, Anhui Institute of Architecture, Hefei 230022, China |
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| Abstract: | The two-phase system medium for Mycobacterium sp.BD696-6 to produce Androst-4-ene-3,17-dione(AD)by degrading sterols was optimized. The obtained optimal medium ingredients were: sunflower oil 20%, molasses 6%, NH4NO3 0.3%, (NH4)2HPO4 0.08%. Under the optimal medium determined, the conversion rate reached to 78.6% when the concentration of substrate steroid was 0.6%, which is17.0% higher than the original medium. |
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| Keywords: | Mycobacterium sp sterols two-phase system medium optimization |
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