酪蛋白糖巨肽(CGMP)对小鼠肠道菌群消长规律的影响

目的:研究了不同剂量的CGMP对小鼠肠道菌群的影响。方法:选用60只BALB/c小鼠(25～30g),随机分成十组(对照组和4～10七个试验组),每组6只;对照1组(日食组)既不灌胃CGMP也不灌胃安慰剂,对照2组灌胃甜乳清粉溶液(0.2ml),对照3组灌胃生理盐水(0.2ml),试验4～10组灌胃等体积(0.2ml)不同浓度的CGMP溶液,灌胃期为15d。分别于灌胃前以及试验的第3、5、7、10、15d和停止灌胃后一周(第22d)用逼迫法采集小鼠新鲜粪便进行菌群测定,观察肠道内菌群的消长规律。结果表明:与对照组相比,随着灌胃时间的延长,各试验组均可促进小鼠肠道和粪便中的双歧杆菌和乳酸杆菌增殖(p<0.01),使致病菌数量明显减少(p<0.05),对肠杆菌、肠球菌数量的影响不明显。CGMP对小鼠肠道菌群的影响作用存在最适剂量(100μg/d),最适剂量组使肠杆菌、肠球菌、致病菌数量明显减少。说明CGMP具有有效地促进和调控小鼠肠道生理性细菌、构筑膜菌群的作用。

Effects of Casein Glycomacropeptidets(CGMP) on Pattern of Growth and Decline of Intestinal Flora in BALB/c Mices

Objective: To investigate the effect of CGMP with different doses on intestinal flora in mice. Methods: 60 BALB/ c mices were divided into ten groups(controls and 4～10 groups).The control 1group was administered only normal fodder. The control 2 group was administered sweet whey at dose of 0.2 ml/d. The control 3 group was received the equal volume of normal saline, and the 4～10 groups were administered the equal volume of CGMP which were of different consistencies for 15d. We analyzed the intestinal flora at 0(before terminating), 3, 5, 7, 10, 15d and 22d (after terminating CGMP for 1 week) and observed the vary regular of the intestinal flora in each group. Results: The number of Bifidobacterium and Lactobacillus increased (p< 0.01), pathogenesis decreased(p<0.05), Enterobacter, Enterococcus had no obvious change on the whole in experimental groups compared with the controls in the intestine and faeces. There is a best dose(100μg/d) in the experiment, and the number of pathogenesis, Enterobacter, Enterococcus decreased obviously in the group 8(100μg/d). This experiment demonstrated that CGMP have had the function of regulating the intestinal flora.