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利用纤维素酶提取笃斯越桔花青素的研究
引用本文:石文娟,林松毅,刘静波,王二雷.利用纤维素酶提取笃斯越桔花青素的研究[J].食品科学,2007,28(11):370-373.
作者姓名:石文娟  林松毅  刘静波  王二雷
作者单位:吉林大学军需科技学院营养与功能食品研究室; 吉林大学军需科技学院营养与功能食品研究室 吉林长春130062; 吉林长春130062;
基金项目:吉林省科技厅应用基础研究项目
摘    要:本实验研究纤维素酶法提取笃斯越桔中的花青素的工艺条件,重点考察了料液比、酶用量、温度、pH及酶解时间等因素对花青素提取率的影响,通过L16(44)正交试验优化出酶法提取的长白山野生笃斯越桔果实中花青素的最佳工艺参数为:料液比1:50,pH为1.5,加酶量2%,温度60℃,酶解时间1h。在此条件下,花青素提取率为6.105%。

关 键 词:笃斯越桔    纤维素酶    花青素  
文章编号:1002-6630(2007)11-0370-04
修稿时间:2007-08-20

Study on Extraction of Anthocyanins in Vaccinium uliginosum L. with Cellulase
SHI Wen-juan,LIN Song-yi,LIU Jing-bo,WANG Er-lei.Study on Extraction of Anthocyanins in Vaccinium uliginosum L. with Cellulase[J].Food Science,2007,28(11):370-373.
Authors:SHI Wen-juan  LIN Song-yi  LIU Jing-bo  WANG Er-lei
Affiliation:Laboratory of Nutrition and Functional Food, College of Quartermaster Technology, Jilin University, Changchun 130062, China
Abstract:Cellulase was used to extract anthocyanins in wild Vaccinium uliginosum L. from Changpbai Mountain area, and then the ratio of solid to liquid, the amount of Cellulase, the temperature, the pH and the time of enzymolysis were investigated emphatically. Through single factors and L16(44) orthogonal design on the extraction process, the optimal parameters were obtained as follows: the ratio of solid to liquid 1:50, pH 1.5, the amount of Cellulase 2%, temperature 60 ℃ and time of enzymolysis 1 h. Based on the above conditions, the extraction rate was 6.105%.
Keywords:Vaccinium uliginosum L    cellulase  anthocyanins
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