动物性食品中六种磺胺类药物残留的快速测定

本研究提出一种快速、准确、同时测定动物性食品中多种磺胺类药物残留的检测方法。样品经乙腈提取、正已烷萃取后,用反相液相色谱法同时测定磺胺、磺胺嘧啶、磺胺二甲嘧啶、磺胺对甲氧嘧啶、磺胺氯哒嗪、磺胺喹恶啉等六种磺胺类药物残留。色谱柱采用KromasilC18柱,流动相为乙腈-0.02%H3PO4(体积比28:72),流速1.0ml/min,检测波长为268nm。回收率在80.9%～93.9%之间,线性范围为2.08～38.0μg/ml(r在0.9921～0.9999之间)。实验结果表明该法快速、简便、准确可靠。

Rapid Determination of Six Kinds of Sulphonamides Residues in Animal Source Food

A method was set up for the simultaneous determination of several kinds of sulphonamides residues, which proved to be rapid and accurate. The sample was extracted by acetonitrile, cleaned by n-hexane. Six sulphonamides residues, such as sulfanilamide, sulfadiazine, sulfamethazine, sulfameter, sulfachloropyridazize and sulfaquinoxaline in animal souce food could be determined by reversed phase high performance liquid chromatography on a kromasil C18 column at 268 nm. Acetonitrile -0.02% H3PO4(V/V 28:72)was used as the mobile phase at a flow rate of 1.0 ml/min. The recovery is 80.9%～93.9%. The linear range is 2.08～38.0μg/ml(r=0.9921～0.9999).