反相高效液相色谱法用于玉竹中黄酮类化合物槲皮素的分离与测定

采用反相高效液相色谱,在C18柱上以乙酸-甲醇-乙腈-磷酸-水为多元流动相,等度洗脱,在30min内对玉竹的黄酮提取物直接进行分离与测定,检测波长为278nm,流速0.50ml/min,采用校准曲线法对实际样品中的槲皮素进行定量分析。实验结果表明:本方法平均加标回收率96.79%,相对标准偏差0.89%,该法简便、准确、快速,可用于玉竹黄酮提取物槲皮素的精确分析。

Separation and Determination of Quercetin of Polygonatum Flavonoids Extract by RP-HPLC

A reversed-phase HPLC method for the separation and determination of quercetin in Polygonatum flavonoids was described. The components can be separated through a C18 analytical column (250×4.6mm i.d., 5μm) in 30 min. The mobile phase was a mixture of acetic acid-methanol-acetonitrile-phosphoric acid-water (10:100:100:10:300, V/V) respectively. The flow rate was 0.50ml/min. UV detection wavelength was performed at 278 nm. External standard was used and the calibration curve showed good linearity in the range of 1.0～20.4mg/L, r 0.99902, with detection limit as 0.4μg/ml (S/N=3). The average recovery is 96.79%. The relative standard deviation of the method is 0.89%. The method is simple, fast, sensitive and accurate. It can be applied to the analysis of quercetin in Polygonatum. It provides a scientific basis for industrial production and quality control of Polygonatum quercetin liquor preparations for clinical uses.