| 反相高效液相色谱法测定笃斯越桔叶片中槲皮素含量 |
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| 引用本文： | 张燕,林松毅,刘静波,王二雷,宋龙凤. 反相高效液相色谱法测定笃斯越桔叶片中槲皮素含量[J]. 食品科学, 2007, 28(11): 404-407 |
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| 作者姓名： | 张燕 林松毅 刘静波 王二雷 宋龙凤 |
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| 作者单位： | 吉林大学军需科技学院营养与功能食品研究室； 吉林大学军需科技学院营养与功能食品研究室 吉林长春130062； 吉林长春130062； |
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| 基金项目： | 吉林省科技厅应用基础研究项目 |
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| 摘 要： | 采用ShimpackVP-ODS色谱柱(4.6mm×250mm,5μm),以甲醇-0.5%磷酸(50/50)为流动相,360nm为检测波长,用RP-HPLC法测定了笃斯越桔叶片中槲皮素的平均含量为0.78%;方法学验证结果为精密度实验RSD=1.13%(n=5),重现性实验RSD=1.73%(n=5),加样回收率平均值Y=101.6%,RSD=1.93%(n=5),说明本方法精密度、重现性、加样回收率均较好。
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| 关 键 词： | 笃斯越桔 槲皮素 RP-HPLC |
| 文章编号： | 1002-6630（2007）11-0404-04 |
| 修稿时间： | 2007-08-05 |
Determination of Queretion in Leaves of Vacciniumu Liginosum L. by RP-HPLC |
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| ZHANG Yan,LIN Song-yi,LIU Jing-bo,WANG Er-lei,SONG Long-feng. Determination of Queretion in Leaves of Vacciniumu Liginosum L. by RP-HPLC[J]. Food Science, 2007, 28(11): 404-407 |
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| Authors: | ZHANG Yan LIN Song-yi LIU Jing-bo WANG Er-lei SONG Long-feng |
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| Affiliation: | Laboratory of Nutrition and Functional Food, College of Quartermaster Technology, Jilin University, Changchun 130062, China |
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| Abstract: | Determination of Quercetin in the leaves of Vacciniumu Liginosum L. was carried out. The main conditions of RP- HPLC were that: the column Shimpack VP-ODS(4.6mm×250mm, 5μm), mobile phase methanol and 0.5% of phosphoric acid (50/ 50), detecting wavelength 360 nm. The average content of quercetin was 0.78%. The results of methodology were: precision test RSD=1.13%(n=5), reproducibility test RSD=1.73%(n=5), average recovery rate of samples Y=101.6%, RSD=1.93% (n=5), which indicated that the precision, reproduction and recovery rate were proper for built of determination method. |
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| Keywords: | RP-HPLC |
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