车前子多糖对骨髓来源树突状细胞表型和吞噬功能的影响

目的:探讨车前子多糖对小鼠骨髓来源树突状细胞(BMDCs)表型和功能的影响。方法:从小鼠骨髓中分离单核细胞,加入细胞因子rmGM-CSF、rmIL-4诱导分化成未成熟树突状细胞,收集细胞加入促成熟刺激剂LPS(阳性对照组)或车前子多糖。倒置显微镜观察树突状细胞的形态,流式细胞术检测成熟树突状细胞的表面标志CD11c和MHCII的表达及吞噬FITC-dextran的变化。结果:经4种不同的车前子多糖作用40h后,显著促进CD11c+MHCII+双阳性细胞的比率,在浓度(0～50μg/ml)范围内呈现剂量依赖性,当浓度高于50μg/ml时,双阳性细胞表达率呈现降低趋势。经多糖作用后吞噬FITC-dextran的能力降低,其中PS3的作用效果最明显,表达PE-CD11c×FITC-dextran的细胞比率只有11.53%。结论:车前子多糖促进CD11c+MHCII+双阳性细胞的比率,降低对FITC-dextran的吞噬能力,初步表明车前子多糖可以促进树突状细胞的成熟。

Effects of Semen Plantaginis Polysaccharides on Phenotypic and Endocytosis of Murine Dendritic Cells

Objective: To investigate the effects of Semen Plantaginis polysaccharides on the phenotypic and endocytosis of murine dendritic cells. Methods: Imature BMDCs were generated in vitro from the murine bone marrow cells in the presence of rmGM-CSF and rmIL-4, while mature DCs were obtained by cultivation of immature DCs with LPS or polysaccharides. We analyzed the morphological characterization under microscopy, analyzed the cell surface marker of CD11c and MHC II and uptake FITC-dextran by flow cytometric. Results: Treatment with polysaccharides for 40h resulated in the enhanced cell-surface expression of CD11c and MHC II on DCs, and at the the concentration (0～50μg/ml) was in a does-dependent up-regulation the percentage of CD11c and MHC II double positive DCs. And the endocytosis was decrease compared with untreated. Especially, PS3 had a significant effect that the percentage of double-stained cells (PE-CD11c×FITC-dextran) was 11.53%. Conclusions: The Semen Plantaginis polysaccharides could up-regulation the percentage of CD11 and MHC II double positive DCs, and decresae the endocytosis, which indicated that the polysaccharides could induce maturation of murined endritic cells.