| 酸性染料比色法测定两面针药材总生物碱的含量 |
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| 引用本文： | 龙盛京,陈瑶,彭兴.酸性染料比色法测定两面针药材总生物碱的含量[J].食品科学,2007,28(11):443-445. |
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| 作者姓名： | 龙盛京 陈瑶 彭兴 |
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| 作者单位： | 广西医科大学化学教研室； 广西医科大学化学教研室 广西南宁530021； 广西南宁530021； |
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| 摘 要： | 以氯化两面针碱为标样,采用溴麝香草酚蓝为酸性染料,在总生物碱与其形成稳定络合物时间范围内,利用比色法测定两面针药材中总生物碱的含量,测定波长为393nm。两面针总生物碱检测浓度在2.56～10.26μg/ml范围内线性关系良好(r=0.9997),平均回收率为101.00%(RSD=2.46%,n=6)。该法测定两面针总生物碱含量简便快捷,准确易行。
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| 关 键 词： | 两面针 总生物碱 酸性染料比色法 |
| 文章编号： | 1002-6630（2007）11-0443-03 |
| 修稿时间： | 2006-03-29 |
Determination of Total Alkaloid in Zanthoxylum nitidum(Roxb.)DC, by Bromophenol Blue Colorimetry |
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| LONG Sheng-jing,CHEN Yao,PENG Xing.Determination of Total Alkaloid in Zanthoxylum nitidum(Roxb.)DC, by Bromophenol Blue Colorimetry[J].Food Science,2007,28(11):443-445. |
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| Authors: | LONG Sheng-jing CHEN Yao PENG Xing |
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| Affiliation: | Department of Chemistry, Guangxi Medical University, Nanning 530021, China |
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| Abstract: | The content of total alkaloid in Zanthoxylum nitidum(Roxb.) was determined by bromophenol blue colorimetry, and the UV-visible spectrophometry was adopted to determine the total alkaloid with the wavelength at 393 nm. The total alkaloid is in linear relationship within the concentration range of 2.56～ 10.26μg/ml(r=0.9997), the average recovery rate is 101.00% (RSD=2.46%, n=6). Bromophenol blue colorimetry is simple, quick and accurate in the determination of total alkaloid in ZanthoxyLum nitidum (Roxb.)DC. |
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| Keywords: | ZanthoxyLum nitidum(Roxb )DC total alkabid bromophenol blue colorimetry |
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