碱液和木瓜蛋白酶预处理对葛仙米多糖提取效果的影响

多糖的保健功能日益得到学术界的重视与证实。葛仙米中含有丰富的多糖。以碱液和木瓜蛋白酶对葛仙米进行预处理,正交试验确定最佳预处理工艺。多糖提取条件为料水比1:300,于90℃水浴提取2次,2h/次。结果说明,以木瓜蛋白酶预处理葛仙米,按100mg葛仙米加入10ml水充分吸胀后,加入含1.5mg木瓜蛋白酶的缓冲液,在50℃的水浴条件下,经过10h预处理后,葛仙米多糖得率最高,为29.53%。以NaOH溶液预处理葛仙米,最佳的条件是0.4mol/L的浓度,于40℃处理2h,多糖得率可高达35.15%。将碱与酶结合使用,则多糖的得率可以高达47.56%,比对照高出25.12%。

Effects of Alkali and Papain Pretreatment on Nostoc sphaeroids kutz Polysaccharides Extraction

The health protection function of polysaccharides has been payed attention and affirmed by the sphere of leaning. Polysaccharides is rich in Nostoc sphaeroids kutz. Nostoc sphaeroids kutz was pretretmented with alkali and papain, and the best pretreatment method was defined with the orthogonal test. The condition of the extracting polysacchariedes are that the material hydraulic proportion 1: 300, extracted 2 times at 90℃ water baths for 2 h. The results showed: the optimal enzyme pretreatment conditions are 10 mg papain added to the 100 mg Nostoc sphaeroids kutz material, pretreatmented for 10 h at 50 ℃. The polysaccharides is 29.53%; The optimal alkali treatment conditions are the NaOH content 0.4 mol/L, pretreatmented for 2 h at 40 ℃. The polysaccharides yield is 35.15%. Pretreatmenting Nostoc sphaeroids kutz with alkali and papain, the highest polysaccharides yield is 47.56%, higher 25.12% than the contrast.