| 蜂蛹超氧化物歧化酶(SOD)的提取及理化性质研究 |
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| 引用本文： | 张海生,陈锦屏,武巧莉,陈蕾. 蜂蛹超氧化物歧化酶(SOD)的提取及理化性质研究[J]. 食品科学, 2007, 28(10): 192-195 |
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| 作者姓名： | 张海生 陈锦屏 武巧莉 陈蕾 |
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| 作者单位： | 陕西师范大学食品工程系； 陕西师范大学化学与材料科学学院； 华南理工大学轻工与食品学院 陕西西安710062； 陕西西安710062； 广东广州510641； |
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| 摘 要： | 对蜂蛹超氧化物歧化酶(SOD)的提取工艺及理化性质进行了研究。结果表明,用磷酸盐缓冲液可以对蜂蛹SOD进行有效的提取,最佳提取工艺条件为:用pH7.6的磷酸盐缓冲液于室温下静置提取4h后于55℃条件下加热20min,按该工艺条件进行提取,蜂蛹SOD的活力为110.41U/mgpro。蜂蛹SOD最适温度为40～60℃,最适pH为pH7.0～9.0,氰化钾和H2O2是蜂蛹SOD的抑制剂,可以有效地抑制蜂蛹SOD的活性。
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| 关 键 词： | 蜂蛹 SOD 提取 理化性质 |
| 文章编号： | 1002-6630（2007）10-0192-04 |
| 修稿时间： | 2007-06-23 |
Study on Extraction and Physical-chemical Properties of Superoxide Dismutase from Honeybee Pupae |
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| ZHANG Hai-sheng,CHEN Jin-ping,WU Qiao-li,CHEN Lei. Study on Extraction and Physical-chemical Properties of Superoxide Dismutase from Honeybee Pupae[J]. Food Science, 2007, 28(10): 192-195 |
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| Authors: | ZHANG Hai-sheng CHEN Jin-ping WU Qiao-li CHEN Lei |
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| Affiliation: | 1.Department of Food Engineering, Shaanxi Normal University, Xi’an 710062, China; 2.School of Chemistry and Materials Science, Shaanxi Normal University, Xi’an 710062, China; 3.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China |
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| Abstract: | The extraction technology and physical-chemical properties of honeybee pupae SOD were studied in this research. The results showed that SOD could be effectively extracted from honeybee pupae with phosphate buffer and the optimum extraction technology was showed as following: the pH of phosphate buffer pH7.6, the extraction time 4 h, at room temperature, the heating temperature 55 ℃, and heating time 20 min.With this technology, the activity of honeybee pupae SOD was 110.41 U/mg pro. The optimum temperature of honeybee pupae SOD ranged from 40 ℃ to 60 ℃ and the optimum pH scope was pH7. 0～9.0. Potassuim cyanide and H2O2 were the inhibitor of honeybee pupae SOD and they could effectively prevent SOD activity. |
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| Keywords: | honeybee pupae SOD extraction physical-chemical property |
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