用通用引物扩增细胞色素b基因进行牦牛肉的鉴定

本研究的目的是建立牦牛和黄牛肉的基因水平鉴定方法。从牦牛、黄牛和四川几个地方黑山羊品种的肉中提取基因组DNA,采用一对通用引物扩增细胞色素b基因中一段长度为421bp的序列,并进行了克隆测序。结果显示,通用引物能很好地扩增不同动物细胞色素b基因,扩增片段序列在牦牛与黄牛之间存在差异较大,能用于这两种肉的鉴定;而在金堂黑山羊、白玉黑山羊和美姑黑山羊之间差异非常小。本研究建立了PCR-RFLP方法鉴定牦牛和黄牛肉,这对于动物保护以及来源不明的肉类产品的区分具有实际应用价值。

Identification of Yak Meat by Amplification of Cytochrome b Gene with Universal Primers

The objective of this research was to develop a method to identify yak and bovine meat by gene analysis. Genomic DNA was extracted from meat samples of yak, bovine and local goat breeds of Sichuan Province. A universal PCR primer was used to amplify a 421bp fragment of cytochrome b gene for cloning and sequencing. Cytochrome b genes from different animals were amplified efficiently with the universal PCR primer. The amplified fragments show significant differences in sequence between yak and bovine, and can be employed for identification of the two kinds of meat. However, Jintang black goat, Baiyu black goat and Meigu black goat exhibit very small difference in the amplified sequence. A PCR-RFLP based protocol was developed in this experiment to identify yak and bovine meat or meat product, with application value for animal protection and identification of meat products of unknown origin.