Vascular rejection post heart transplantation is associated with positive flow cytometric cross-matching |
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| Authors: | JF McCarthy DJ Cook MG Massad Y Sano KJ O'Malley NR Ratliff RW Stewart NG Smedira RC Starling JB Young PM McCarthy |
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| Affiliation: | Dipartimento di Sanita', Patologia, Farmaco-Tossicologia e Benessere Animale, Facolta' di Medicina Veterinaria, Valenzano, Bari, Italy. |
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| Abstract: | 28 isolates of canine parvovirus type-2 (CPV-2) were obtained from dogs with hemorrhagic gastroenteritis in Italy. The antigenic structure of CPV-2 isolates was characterized, using four discriminating monoclonal antibodies. In addition, four vaccinal strains were examined. Similar to reports from Australia and the United Kingdom, a much higher prevalence of CPV-2a (25/28 isolates) was observed than the other variant type, CPV-2b (3/28 isolates). DNA fragments (2.2 kbp) of representative strains of CPV-2, CPV-2a and CPV-2b were amplified by the polymerase chain reaction (PCR) and the products were digested by the restriction enzymes (RE) RsaI, HpaII, HindIII and PvuII. The RvaI enzyme allows the differentiation of CPV-2 from CPV-2a and CPV-2b. |
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