High performance reversed phase chromatography of cholesterol and cholesteryl esters of human plasma lipoproteins |
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| Authors: | Edward G Perkins David J Hendren John E Bauer Ali H El-Hamdy |
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| Affiliation: | (1) Department of Food Science, Burnsides Research Laboratory, University of Illinois, 1208 W. Pennsylvania Ave., 61801 Urbana, IL;(2) Department of Metabolism, College of Veterinary Medicine, University of Florida, 32610 Gainesville, FL |
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| Abstract: | Cholesterol and cholesteryl esters were separated according to their carbon number and number of double bonds by high performance
reversed-phase chromatography (HPRC) using acetonitrile/chloroform/methanol (1∶1∶1, v/v) as a mobile phase. It was found that
within the same equivalent carbon number (ECN) category, cholesterol esters with the highest number of double bonds eluted
ahead of those with a lower number of double bonds, and with thecis isomers eluting ahead of theirtrans partners. Thus, cholesteryl oleate (C27-18∶1c) elutes ahead of cholesteryl palmitate (C27-16∶0) and ahead of cholesteryl elaidate (C27-18∶1t). Human lipoprotein, as well as rat liver cholesteryl esters, were separated using this technique. |
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