A Glutathione Peroxidase Gene from Litopenaeus vannamei Is Involved in Oxidative Stress Responses and Pathogen Infection Resistance |
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| Authors: | Jinquan Fan Binbin Li Qianming Hong Zeyu Yan Xinjun Yang Kecheng Lu Guoliang Chen Lei Wang Yihong Chen |
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| Affiliation: | 1.Key Laboratory for Healthy and Safe Aquaculture/Institute of Modern Aquaculture Science and Engineering (IMASE), College of Life Science, South China Normal University, Guangzhou 510631, China; (J.F.); (B.L.); (Q.H.); (Z.Y.); (X.Y.); (K.L.); (G.C.);2.Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China |
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| Abstract: | In shrimp, several glutathione peroxidase (GPX) genes have been cloned and functionally studied. Increasing evidence suggests the genes’ involvement in white spot syndrome virus (WSSV)- or Vibrio alginolyticus-infection resistance. In the present study, a novel GXP gene (LvGPX3) was cloned in Litopenaeus vannamei. Promoter of LvGPX3 was activated by NF-E2-related factor 2. Further study showed that LvGPX3 expression was evidently accelerated by oxidative stress or WSSV or V. alginolyticus infection. Consistently, downregulated expression of LvGPX3 increased the cumulative mortality of WSSV- or V. alginolyticus-infected shrimp. Similar results occurred in shrimp suffering from oxidative stress. Moreover, LvGPX3 was important for enhancing Antimicrobial peptide (AMP) gene expression in S2 cells with lipopolysaccharide treatment. Further, knockdown of LvGPX3 expression significantly suppressed expression of AMPs, such as Penaeidins 2a, Penaeidins 3a and anti-lipopolysaccharide factor 1 in shrimp. AMPs have been proven to be engaged in shrimp WSSV- or V. alginolyticus-infection resistance; it was inferred that LvGPX3 might enhance shrimp immune response under immune challenges, such as increasing expression of AMPs. The regulation mechanism remains to be further studied. |
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| Keywords: | Litopenaeus vannamei glutathione peroxidase oxidative stress Antimicrobial peptide genes |
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