| 转RIPs基因甜菜的分子检测和抗病性鉴定 |
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| 引用本文： | 马天婕,孙亚卿,史树德,张少英.转RIPs基因甜菜的分子检测和抗病性鉴定[J].中国甜菜糖业,2008(4):1-2,6. |
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| 作者姓名： | 马天婕 孙亚卿 史树德 张少英 |
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| 作者单位： | 内蒙古农业大学甜菜生理研究所,内蒙古,呼和浩特,010018 |
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| 基金项目： | 国家自然科学基金，国家自然科学基金 |
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| 摘 要： | 对利用农杆茵介导法转化的经卡那霉素筛选的转核糖体失活蛋白(RIPs)基因甜菜植株进行PCR和RT-PCR检测,并对分子检测呈阳性的转基因甜菜植株接种甜菜褐斑病菌,接种20d后,发现75%植株具有抗病性.
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| 关 键 词： | 转基因甜菜 RIPs基因 分子检测 抗病性 |
Analyses of resistance and transgenes detection for transgenic sugar beet |
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| MA Tian-jie,SUN Ya-qing,SHI Shu-de,ZHANG Shao-ying.Analyses of resistance and transgenes detection for transgenic sugar beet[J].China Beet & Sugar,2008(4):1-2,6. |
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| Authors: | MA Tian-jie SUN Ya-qing SHI Shu-de ZHANG Shao-ying |
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| Affiliation: | MA Tian-jie,SUN Ya-qing,SHI Shu-de,ZHANG Shao-ying (Institute of Sugarbeet Physiology,Inner Mongolia Agricultural University,Inner Mongolia Huhhot 010018,China) |
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| Abstract: | RIPs gene were transformed into sugar beet using agrobacterium tunfaciens mediated method.Kanamycin resistance regenerate plant is detected by PCR and RT-PCR.Infection experiment showed that resistance of a transgenic plant are obviously higher than CK. |
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| Keywords: | transgenic sugar beet RIPs gene detection resistance |
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